Model Number:

The MicroSprayer®/Syringe Assembly – MSA-250-M for Mouse consists of the MicroSprayer® Aerosolizer – Model IA-1C-M and the FMJ-250 High Pressure Syringe, which is required for use of the Model IA-1C devices. The MicroSprayer® Aerosolizer – Model IA-1C-M is designed for fast, targeted, air-free intratracheal aerosol administration to mouse. The device can be used to administer a precisely quantifiable, highly-concentrated, air-free plume of liquid aerosol directly into the lungs or other locations. It may also be used for intranasal or in vitro applications. It can aerosolize a wide range of liquids or particle solutions/suspensions, including pharmaceutical and biologic formulations, contrast/imaging agents and toxicological materials. (It does not administer dry powders.)

For intrapulmonary applications, the tip of the device is gently inserted down the trachea of the anesthetized animal – near to, but not touching the carina (first bifurcation). The portion that is inserted into the trachea measures 1.25″.

  • Quick, precise aerosol dosing directly to the lungs – with no waste
  • Purely mechanical, air-free technology
  • Uses no heat, propellant, ultrasound or compressed air. No air burden to subject.
  • Unique patented design is light, hand-held, easy to use
  • Rounded tip permits safe, gentle intratracheal insertion
  • Fully sterilizable, autoclavable and reusable
  • Delivers a far higher volume and concentration than is possible with nebulizer/inhalation systems
  • Produces a far broader, more uniform distribution than liquid bolus/droplet instillation methods

MicroSprayer® Aerosolizer – Model IA-1C-M

  • Materials: Based on flexible, 23-gauge stainless steel tubing and other chemically resistant components
  • 120-degree bend: All standard devices made with a 120-degree bend to make it easier to see around one’s hand when trying to view the epiglottis. (Straight custom devices can be ordered.)
  • Length of intratracheal portion: 1.25″
  • Hub: 1/4-28 tpi
  • Tip is rounded to avoid harm to delicate tissues during insertion.
  • Operates only with: Penn-Century’s patented FMJ-250 High Pressure Syringe (REQUIRED)
  • Dose range: When used with FMJ-250 High Pressure Syringe, can administer dose volumes up to 250 microliters, or multiple smaller doses of 25 to 50 microliters or combinations of those dose volumes, using dosing spacers provided.
  • Outer diameter: .025″ (0.64 mm), except at the very tip which is .028″ (0.71mm)
  • Inner diameter: .017″ (0.43 mm) (NOTE: Liquid passes through patented aerosol components at the very tip. The diameter of these passageways in the Model IA-1C is approximately 60 microns in diameter. )
  • Re-usable/Sterilizable: Can be cleaned and sterilized with very hot (filtered or distilled) water, organic solvents, by autoclave or ultrasound.

FMJ-250 High-Pressure Syringe

Required for use with MicroSprayer® Aerosolizer – Model IA-1C

  • Description: Unique patented air-free, gas tight syringe
  • Pressure: Operates at up to 3000 psi.
  • Hub: 1/4-28 tpi (Note: Not a luer hub)
  • Materials: Constructed of stainless steel and chemically-resistant components with a replaceable plunger with a Teflon® tip. May be used with any length Model IA-1C.
  • Maximum Capacity: 250 µl (microliters).
  • Dose increments: Two sets of volume spacers are provided with each syringe. These are attached to the plunger, and removed one by one, per instructions provided, to permit precise single doses of either 25-µl or 50-µl, or combinations of those amounts. Syringe holds multiple doses.
  • Re-usable/Sterilizable: Can be cleaned and sterilized with hot water, organic solvents, or by autoclave or ultrasound.

When analyzed with water, using Penn-Century’s FMJ-250 High Pressure Syringe, the MicroSprayer® Aerosolizer – Model IA-1C produced a particle size distribution with a Mass Median Diameter (MMD) of 16-22 µm (microns) – when analyzed by laser defraction. NOTE: Results may vary, depending upon method of particle size analysis used, the material delivered and the length of the MicroSprayer® Aerosolizer.

The following devices are helpful when working with the MicroSprayer® Aerosolizer – Model MSA-250 in Mouse. The Microsprayer® Aerosolizer Pulmonary Aerosol Kit for Mouse (PAK-MSA), is also available and includes everything you need for a liquid intratracheal aerosol study in mice, at a discounted price.

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Dimensions (approx.)
1.25″ 120° 0.5″

Length “A”: Functional length, from bend to tip
Angle “B”: Angle of bend
Length “C”: Length from hub to bend

Model IA-1C diagram

For intratracheal applications in small animals, the MicroSprayer® Aerosolizer Model IA-1C is usually inserted directly into the trachea. The intratracheal portion of the Model IA-1C is designed to reach from the front incisors to the carina in a standard laboratory mouse. NOTE: Users may also pre-intubate the animal and insert the device via an endotracheal tube. Doing so requires that the user calculate the measurements carefully:

  • If the Model IA-1C is inserted into an endotracheal tube, the distal tip must emerge slightly (approximately 1 cm) from the end of the endotracheal tube when it is in position
  • To avoid injury, neither the distal tip of endotracheal tube nor the distal tip of MicroSprayer® Aerosolizer should make contact with the carina when the plunger of the syringe is pushed

Intratracheal use of Penn-Century devices
For best results, position the tip
of the device near to – but not
touching the carina, or first

Intratracheal use of Penn-Century devices

The process of intratracheal administration is essentially a form of intubation. For intrapulmonary applications, the tip of the device is gently inserted down the trachea of the anesthetized animal – near to, but not touching the carina (first bifurcation).

For planning help, see our Instructions for Use of the MicroSprayer® Aerosolizer – MSA-250.