The Dry Powder Insufflator™ – Model DP-4 is available in two standard sizes – for intratracheal use in rat or guinea pig – or in custom sizes in any length of your choice. Custom versions of the Model DP-4 can be straight or have a 120-degree bend. The delivery tube may be made of rigid stainless steel or flexible plastic tubing, depending on your intended use.
Custom devices can be made to order for special, non-standard intratracheal or intranasal, in vivo or ex vivo applications, or for in vitro experimental laboratory set-ups. Some examples include custom-length devices that are long enough for intratracheal use (by inserting via an endotracheal tube or bronchoscope) in: rabbits, ferrets, dogs, cats, chickens, pigs, sheep, monkeys, horses, etc. Other examples include use for in vitro aerosol particle testing or cell culture exposure.
- To help us understand what device and length will best serve your needs, please complete and submit the Custom Device Questionnaire.
- To help you determine measurements for a custom length Model DP-4, click on the ‘Diagram’ tab on this page or download: Guide to Measuring a Custom-length Dry Powder Insufflator™ – Model DP-4
- For planning information on using of a custom length Model DP-4, see: Instructions for use of Penn-Century Dry Powder Insufflator™ – Model DP-4
The Dry Powder Insufflator™ – Model DP-4-CSS is designed for rapid, targeted, intratracheal powder administration. For pulmonary use, the very tip of the delivery tube must be positioned near but not touching the carina (first bifurcation) in an anesthetized animal. The device exerts a slight insufflating effect, permitting a precisely quantifiable dose of particles to penetrate deep into the lung. It may also be used for intranasal or in vitro applications. The device twists open at the center to reveal a sample chamber that can be filled manually with a small single dose (1 – 5 mg) of powdered material, protected from ambient air and moisture. (Larger doses possible with extension sold separately.) The Model DP-4-CSS operates with a 3 ml disposable air syringe (provided) or, for more rapid administration of air with less force and hand motion, a Penn-Century Air Pump. Dry methods of cleaning the device are preferred.
- Light, easy-to-use, hand-operated pulmonary drug delivery device
- Produces a precisely measurable cloud of fine particles from the end of a hollow, stainless steel delivery tube
- Can be used with powders of a wide range of sizes and characteristics from nanoparticles to large porous particles
- Powders are unaffected by passage through the device
- Widely tested with a range of pharmaceutical and bioactive compounds, as well as radiographic formulations.
- Also useful for testing and development of dry powder formulations – time-of-flight, PK/PD, etc.
- Widely used for occupational, environmental, bioterror and biohazard toxicology studies
- Must be weighed before and after loading and after discharging for precise quantification of delivered dose
- Sample chamber holds powder securely to prevent sample loss, moisture or contamination
- Reusable and sterilizable. Made of virtually indestructible, heat and chemically resistant PEEK™ (polyetheretherketone) plastic and detachable stainless steel delivery tube.
- Description: For fast, precise, targeted administration of a “cloud” of dry powder. Body of device made of chemically resistant PEEK™ plastic (polyetheretherketone) and silicone valves.
- Body of Dry Powder Insufflator™: Made of chemically resistant PEEK™ plastic (polyetheretherketone) and silicone valves
- Delivery tube: Rigid, hollow stainless steel 18-gauge tubing with rounded tip – straight or with 120-degree bend
- Length of intratracheal portion: Determined by the user
- Outer diameter: .050″ (1.1 mm) except at rounded tip which is .058″
- Inner diameter: .038″ (0.9652 mm) hollow throughout
- Single dose range: 1-5 mg (Must be loaded with ONLY ONE dose at a time)
- For larger single doses: 100 mg Sample Chamber Extension may be attached for larger single doses.
- Operates with: Commercial 3ml plastic air syringe (provided). Can also be operated with a Penn-Century Air Pump for more rapid administration of air pulses of 0-5 ml. (100% dose delivery may require more than one pulse of air.)
- Particle size range: Dry Powder Insufflator™ can be loaded with dry powders in a very wide range of sizes from nanoparticles to large macroparticles. Device does not filter or affect powders traveling through it.
- Weight: 3.5 g empty weight, with standard delivery tube
- Precision balance scale required: For precise quantification of the delivered dose, users will require a precision balance
- Reusable/Sterilizable: Stainless steel tip detachable, cleanable with liquid methods, and autoclavable. NOTE: Dry methods of cleaning are preferred for main PEEK™ portions of device
The Penn-Century Dry Powder Insufflator™ has been successfully used to aerosolize powders of a wide range of sizes and characteristics from nanoparticles to large porous particles. The device does not filter or affect the powder sample in any way. If the powder formulation is particularly likely to absorb ambient moisture, the device may be loaded in an air-tight glove bag. Once it is in the sample chamber, it is sealed off from ambient room air and humidity.
The following devices are helpful when working with the Dry Powder Insufflator™ – Model DP-4-C for Custom Lengths:
- Small Animal Laryngoscope – Model LS-2-C for Larger Species
Model Number: LS-2-C Instructions for use Description Specifications Diagram User Info Penn-Century’s Small Animal Laryngoscope Model LS-2-C is specifically designed for custom applications in laboratory animals larger than mouse, rat or guinea pig – such as rabbit, ferret or small primates. (Please contact us to discuss your requirements.) The device provides bright, clear illumination for… Continue reading
- Small Animal Laryngoscope – Model LS-2-C for Larger Species
- Sample Chamber Extension – Model SC-X
Model Number: SC-X Description Specifications Diagram User Info The Dry Powder Insufflator™ – Model DP-4 has a standard dose volume capacity of 1-5 mg. The 100 mg Sample Chamber Extension (SC-X) is designed to provide additional capacity for larger dose volumes when used with the Dry Powder Insufflator™ – Model DP-4. It is a hollow… Continue reading$98.00
- Sample Chamber Extension – Model SC-X
Delivery tube of Dry Powder Insufflator™ – Model DP-4-C for Custom Lengths
For custom length, straight devices, the length “A” must be specified, as shown:
Length “A”: Functional length, from bend to tip
Angle “B”: Angle of bend
Length “C”: Length from hub to bend
For intratracheal applications with the Dry Powder Insufflator™, optimal concentration and uniform deposition in the lung is achieved when the tip of the device is close to but not touching the carina. The intratracheal portion of the Model DP-4-C is designed to be inserted directly into the trachea and reach from the front incisors to the carina.
Users may also pre-intubate the animal and insert the device down the trachea to the carina via an endotracheal tube. Doing so requires that the user calculate the measurements carefully:
- If tip of the Dry Powder Insufflator™ is inserted into an endotracheal tube, the distal tip must emerge slightly from the end of the endotracheal tube when it is in position
- To avoid injury, neither the distal tip of endotracheal tube nor the distal tip of the Dry Powder Insufflator™ should make contact with the carina when the plunger of the air syringe is pushed
Intratracheal use of Penn-Century devices
For best results, position the tip
of the device near to – but not
touching the carina, or first
The process of intratracheal administration is essentially a form of intubation. For optimal powder deposition and distribution in the lung, the tip of the device is gently inserted down the trachea of the anesthetized animal – near to, but not touching the carina (first bifurcation). Care must be taken to avoid unnecessary hand motion to avoid injury to the animal.
For complete details, please refer to the Instructions for Use of the Penn-Century Dry Powder Insufflator™ – Model DP-4
- A precision balance is required to quantify the dose.
Use of the Dry Powder Insufflator™ – Model DP-4 requires a precision scale or balance. The device must be precisely weighed before and after filling and after each application of air to quantify the delivered dose and to determine how many applications of air will be required to dispense 100% of that dose.
- Insufflation of the powder sample.
The Model DP-4 is operated with either a 3 ml commercial air syringe (provided) or a Penn-Century Air Pump (sold separately.) The air source is firmly press-fit into the proximal end of the Air Handler to insufflate the powder sample with puffs or pulses of air. NOTE: To avoid injury and hyperinflation to the experimental animal, the volume of air in the air source must be set to the correct volume for the lung capacity of the experimental animal. For rat, air volumes must not exceed 2 ml. For guinea pig, air volumes must not exceed 3 ml. Some powder formulations may require more than one application of air to dispense 100% of the powder.
- Positioning of the delivery tube in the trachea.
For optimal deposition and distribution in the lung, the very tip of the Dry Powder Insufflator™ must be carefully positioned in the trachea of the anesthetized animal so that the tip of the delivery tube is above, but not touching the carina, as indicated in the drawing (right). To insufflate the powder with an air syringe requires the user to push the plunger rapidly and with force. At the same time, great care must be taken, when pushing the plunger of the air syringe, to avoid excessive hand motion that may result in pushing the tip of the device forward and causing injury to the animal. If the user is using a spring-loaded Penn-Century Air Pump instead of the air syringe, less force and hand motion is required to produce a puff of air of a specific volume. For detailed technical information, refer to the Instructions for Use.
- Cleaning of the main device body – Dry methods preferred.
In general, the Dry Powder Insufflator™ is constructed of sterilizable materials. Due to the complex nature of the valve system inside, dry methods of cleaning are preferred. Liquid methods of cleaning the device, such as alcohol, organic solvents (ethanol or methanol), or water are less desirable than physical methods. Liquids, especially water, may work themselves into small crevices where they may interfere with subsequent powder deliveries. (Please note that dessication systems are designed to remove water – not solvents.) To clean the Sample Chamber, detach it from the Air Intake and direct a small stream of air or other compressed gas (“Dust-Off” or a similar product for cleaning computer keyboards will do). Take care not to thrust the nozzle of the air source into the Sample Chamber to avoid damage to the valve assembly. Do not use excessively forceful compressed air sources. If you have particular questions or concerns about acceptable methods of cleaning, feel free to contact us with your questions.
- Cleaning the stainless steel delivery tube.
The delivery tube of the device is hollow and detachable, and may be cleaned separately. The device may be autoclaved or immersed in water, organic solvent or disinfectant. It should be thoroughly and completely dried – using compressed air or by air drying it, prior to reattaching to the body of the device. To remove the delivery tube, simply twist the 10-32 fitting 1/4-turn counterclockwise and pull the tube out. To replace, push the stainless steel tube in to a hard stop and twist the fitting 1/4-turn clockwise, or until some resistance is felt. Do not overtighten.